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Jiancheng Inc creatinine cr assay kit
Creatinine Cr Assay Kit, supplied by Jiancheng Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Viability Assay:

Article Title: Overcoming ADC resistance in advanced colorectal cancer by dual targeting of TROP2 and PERK to suppress Wnt/β-catenin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-TROP2 Abcam Cat#AB214488; RRID:AB_2811182 Rabbit monoclonal anti-Ki67 Cell Signaling Technology Cat#9027; RRID:AB_2636984 Rabbit monoclonal anti-PERK Cell Signaling Technology Cat#5683; RRID:AB_10841299 Rabbit monoclonal anti-Phospho-PERK/ EIF2AK3 Proteintech Cat#82534-1-RR; RRID:AB_3085359 Rabbit monoclonal anti-eIF2α Proteintech Cat#11170-1-AP; RRID:AB_2096489 Rabbit monoclonal anti-Phospho-eIF2α Proteintech Cat#68023-1-Ig; RRID:AB_2918767 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat#11815S; RRID:AB_2616025 Rabbit monoclonal anti-β-Catenin Proteintech Cat#51067-2-AP; RRID:AB_2086128 Rabbit polyclonal anti-Jun Proteintech Cat#24909-1-AP; RRID:AB_2860574 Mouse polyclonal anti-GSK3B Proteintech Cat#67329-1-Ig; RRID:AB_2882588 Rabbit polyclonal anti-WNT2B ABclonal Cat#A19554; RRID:AB_3741590 Rabbit monoclonal anti-GAPDH Proteintech Cat#81640-5-RR; RRID:AB_3086559 Rabbit monoclonal anti-Phospho-Beta Catenin (Ser33) Proteintech Cat#80067-1-RR; RRID:AB_2918861 Rabbit monoclonal anti-Phospho-GSK-3 beta (Ser9) (5B3) Cell Signaling Technology Cat#9323; RRID:AB_2115201 Rabbit monoclonal anti-Cyclin D1 (E3P5S) Cell Signaling Technology Cat#55506; RRID:AB_2827374 Rabbit polyclonal anti-c-MYC Proteintech Cat#10828-1-AP; RRID:AB_2148585 Rabbit monoclonal anti-Phospho-Histone H2A.X (Ser139) (20E3) Cell Signaling Technology Cat#9718; RRID:AB_2118009 Rabbit polyclonal anti-TOP1 Proteintech Cat#20705-1-AP; RRID:AB_10695762 Rabbit polyclonal anti-TCF1 (C63D9) Cell Signaling Technology Cat#2203; RRID:AB_2199302 HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat#RGAR001; RRID:AB_3073505 HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat#RGAM001; RRID:AB_3068333 Biological samples CRC patient samples This paper Table S1 Chemicals, peptides, and recombinant proteins IMMU132 Gilead Sciences Cat#S22F026D Capecitabine Selleck Cat#S1156 Oxaliplatin Selleck Cat#S1224 GSK2606414 Selleck Cat#S7307 GSK2656157 Selleck Cat#S7033 AMG44 Selleck Cat#E1761 PERK-IN-4 MedChemExpress Cat#1337531-89-1 MSAB Selleck Cat#S6901 SKL2001 Selleck Cat#S8320 Polybrene YEASEN Cat#40804ES76 Puromycin Beyotime Cat#ST551 SDS Lysis Buffer Beyotime Cat#P0013G PMSF Solarbio Cat#P0100 Phosphatase inhibitor cocktail Beyotime Cat#P1081 (Continued on next page) Cell Reports Medicine 7, 102769, May 19, 2026 e1 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-fade Mounting Medium with DAPI Beyotime Cat#P0131 Critical commercial assays CellTiter-Glo® Luminescent Cell Viability Assay Promega Cat#G7573 EdU Cell Proliferation Kit with Alexa Fluor 555 EpiZyme Cat#CX003 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062L UltraSensitiveTM SP(mouse/rabbit)IHC Kit MXB Biotechnologies Cat#KIT-9730 TransZol Up TransGen Biotech Cat#ET111 EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech Cat#AE311 PerfectStart® Green qPCR SuperMix TransGen Biotech Cat#AQ602 Colorectal Cancer Organoid Kit BioGenous Technologies Cat#K2103-CR Tumor Tissue Digestion Solution BioGenous Technologies Cat#K601003 Cancer Organoid Basal Medium BioGenous Technologies Cat#B213152 Organoid Culture ECM BioGenous Technologies Cat#M315066 Organoid Dissociation Solution BioGenous Technologies Cat#E238001 Alanine aminotransferase Assay Kit Jiancheng Bioengineering Cat#C009-2-1 Aspartate aminotransferase Assay Kit Jiancheng Bioengineering Cat#C010-2-1 Urea Assay Kit Jiancheng Bioengineering Cat#C013-2-1 Uric acid (UA) Test Kit Jiancheng Bioengineering Cat#C012-2-1 Creatinine (Cr) Assay kit (sarcosine oxidase) Jiancheng Bioengineering CatC011-2-1 α-Amylase Assay Kit Jiancheng Bioengineering Cat#C016-1-2 Lipase assay kit Jiancheng Bioengineering Cat#A054-2-1 Deposited data PDX RNAseq This paper GEO: GSE311016 Cell line RNAseq This paper GEO: GSE312098 Experimental models: Cell lines Human:CX-1 iCell Bioscience Inc. iCell-h050 Human:DLD-1 iCell Bioscience Inc. iCell-h053 Human:HCT15 iCell Bioscience Inc. iCell-h072 Human:COLO205 iCell Bioscience Inc. iCell-h045 Human:HEK293T iCell Bioscience Inc. iCell-h237 Experimental models: Organisms/strains Mouse: NOD/SCID mice females Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Mouse: C57BL/6 mice males Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Oligonucleotides ShPERK#1 targeted sequence: 5′- GAAACAGCTATTCTCATAAAG -3′ Sangon Biotech N/A ShPERK #2 targeted sequence: 5′- GTTGTGCTAGCAACCCTAATA -3′ Sangon Biotech N/A Recombinant DNA pLKO.1 This paper N/A plasmids pMD2.G This paper Addgene plasmid #12259 Plasmids psPAX2 This paper Addgene plasmid #12260 Software and algorithms GraphPad Prism 10.1.2 Graphpad software https://www.graphpad.com ImageJ This paper https://imagej.net/ij/. (Continued on next page) e2 Cell Reports Medicine 7, 102769, May 19, 2026 Article ll OPEN ACCESS ..

cDNA Synthesis:

Article Title: Overcoming ADC resistance in advanced colorectal cancer by dual targeting of TROP2 and PERK to suppress Wnt/β-catenin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-TROP2 Abcam Cat#AB214488; RRID:AB_2811182 Rabbit monoclonal anti-Ki67 Cell Signaling Technology Cat#9027; RRID:AB_2636984 Rabbit monoclonal anti-PERK Cell Signaling Technology Cat#5683; RRID:AB_10841299 Rabbit monoclonal anti-Phospho-PERK/ EIF2AK3 Proteintech Cat#82534-1-RR; RRID:AB_3085359 Rabbit monoclonal anti-eIF2α Proteintech Cat#11170-1-AP; RRID:AB_2096489 Rabbit monoclonal anti-Phospho-eIF2α Proteintech Cat#68023-1-Ig; RRID:AB_2918767 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat#11815S; RRID:AB_2616025 Rabbit monoclonal anti-β-Catenin Proteintech Cat#51067-2-AP; RRID:AB_2086128 Rabbit polyclonal anti-Jun Proteintech Cat#24909-1-AP; RRID:AB_2860574 Mouse polyclonal anti-GSK3B Proteintech Cat#67329-1-Ig; RRID:AB_2882588 Rabbit polyclonal anti-WNT2B ABclonal Cat#A19554; RRID:AB_3741590 Rabbit monoclonal anti-GAPDH Proteintech Cat#81640-5-RR; RRID:AB_3086559 Rabbit monoclonal anti-Phospho-Beta Catenin (Ser33) Proteintech Cat#80067-1-RR; RRID:AB_2918861 Rabbit monoclonal anti-Phospho-GSK-3 beta (Ser9) (5B3) Cell Signaling Technology Cat#9323; RRID:AB_2115201 Rabbit monoclonal anti-Cyclin D1 (E3P5S) Cell Signaling Technology Cat#55506; RRID:AB_2827374 Rabbit polyclonal anti-c-MYC Proteintech Cat#10828-1-AP; RRID:AB_2148585 Rabbit monoclonal anti-Phospho-Histone H2A.X (Ser139) (20E3) Cell Signaling Technology Cat#9718; RRID:AB_2118009 Rabbit polyclonal anti-TOP1 Proteintech Cat#20705-1-AP; RRID:AB_10695762 Rabbit polyclonal anti-TCF1 (C63D9) Cell Signaling Technology Cat#2203; RRID:AB_2199302 HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat#RGAR001; RRID:AB_3073505 HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat#RGAM001; RRID:AB_3068333 Biological samples CRC patient samples This paper Table S1 Chemicals, peptides, and recombinant proteins IMMU132 Gilead Sciences Cat#S22F026D Capecitabine Selleck Cat#S1156 Oxaliplatin Selleck Cat#S1224 GSK2606414 Selleck Cat#S7307 GSK2656157 Selleck Cat#S7033 AMG44 Selleck Cat#E1761 PERK-IN-4 MedChemExpress Cat#1337531-89-1 MSAB Selleck Cat#S6901 SKL2001 Selleck Cat#S8320 Polybrene YEASEN Cat#40804ES76 Puromycin Beyotime Cat#ST551 SDS Lysis Buffer Beyotime Cat#P0013G PMSF Solarbio Cat#P0100 Phosphatase inhibitor cocktail Beyotime Cat#P1081 (Continued on next page) Cell Reports Medicine 7, 102769, May 19, 2026 e1 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-fade Mounting Medium with DAPI Beyotime Cat#P0131 Critical commercial assays CellTiter-Glo® Luminescent Cell Viability Assay Promega Cat#G7573 EdU Cell Proliferation Kit with Alexa Fluor 555 EpiZyme Cat#CX003 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062L UltraSensitiveTM SP(mouse/rabbit)IHC Kit MXB Biotechnologies Cat#KIT-9730 TransZol Up TransGen Biotech Cat#ET111 EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech Cat#AE311 PerfectStart® Green qPCR SuperMix TransGen Biotech Cat#AQ602 Colorectal Cancer Organoid Kit BioGenous Technologies Cat#K2103-CR Tumor Tissue Digestion Solution BioGenous Technologies Cat#K601003 Cancer Organoid Basal Medium BioGenous Technologies Cat#B213152 Organoid Culture ECM BioGenous Technologies Cat#M315066 Organoid Dissociation Solution BioGenous Technologies Cat#E238001 Alanine aminotransferase Assay Kit Jiancheng Bioengineering Cat#C009-2-1 Aspartate aminotransferase Assay Kit Jiancheng Bioengineering Cat#C010-2-1 Urea Assay Kit Jiancheng Bioengineering Cat#C013-2-1 Uric acid (UA) Test Kit Jiancheng Bioengineering Cat#C012-2-1 Creatinine (Cr) Assay kit (sarcosine oxidase) Jiancheng Bioengineering CatC011-2-1 α-Amylase Assay Kit Jiancheng Bioengineering Cat#C016-1-2 Lipase assay kit Jiancheng Bioengineering Cat#A054-2-1 Deposited data PDX RNAseq This paper GEO: GSE311016 Cell line RNAseq This paper GEO: GSE312098 Experimental models: Cell lines Human:CX-1 iCell Bioscience Inc. iCell-h050 Human:DLD-1 iCell Bioscience Inc. iCell-h053 Human:HCT15 iCell Bioscience Inc. iCell-h072 Human:COLO205 iCell Bioscience Inc. iCell-h045 Human:HEK293T iCell Bioscience Inc. iCell-h237 Experimental models: Organisms/strains Mouse: NOD/SCID mice females Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Mouse: C57BL/6 mice males Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Oligonucleotides ShPERK#1 targeted sequence: 5′- GAAACAGCTATTCTCATAAAG -3′ Sangon Biotech N/A ShPERK #2 targeted sequence: 5′- GTTGTGCTAGCAACCCTAATA -3′ Sangon Biotech N/A Recombinant DNA pLKO.1 This paper N/A plasmids pMD2.G This paper Addgene plasmid #12259 Plasmids psPAX2 This paper Addgene plasmid #12260 Software and algorithms GraphPad Prism 10.1.2 Graphpad software https://www.graphpad.com ImageJ This paper https://imagej.net/ij/. (Continued on next page) e2 Cell Reports Medicine 7, 102769, May 19, 2026 Article ll OPEN ACCESS ..

Real-time Polymerase Chain Reaction:

Article Title: Overcoming ADC resistance in advanced colorectal cancer by dual targeting of TROP2 and PERK to suppress Wnt/β-catenin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-TROP2 Abcam Cat#AB214488; RRID:AB_2811182 Rabbit monoclonal anti-Ki67 Cell Signaling Technology Cat#9027; RRID:AB_2636984 Rabbit monoclonal anti-PERK Cell Signaling Technology Cat#5683; RRID:AB_10841299 Rabbit monoclonal anti-Phospho-PERK/ EIF2AK3 Proteintech Cat#82534-1-RR; RRID:AB_3085359 Rabbit monoclonal anti-eIF2α Proteintech Cat#11170-1-AP; RRID:AB_2096489 Rabbit monoclonal anti-Phospho-eIF2α Proteintech Cat#68023-1-Ig; RRID:AB_2918767 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat#11815S; RRID:AB_2616025 Rabbit monoclonal anti-β-Catenin Proteintech Cat#51067-2-AP; RRID:AB_2086128 Rabbit polyclonal anti-Jun Proteintech Cat#24909-1-AP; RRID:AB_2860574 Mouse polyclonal anti-GSK3B Proteintech Cat#67329-1-Ig; RRID:AB_2882588 Rabbit polyclonal anti-WNT2B ABclonal Cat#A19554; RRID:AB_3741590 Rabbit monoclonal anti-GAPDH Proteintech Cat#81640-5-RR; RRID:AB_3086559 Rabbit monoclonal anti-Phospho-Beta Catenin (Ser33) Proteintech Cat#80067-1-RR; RRID:AB_2918861 Rabbit monoclonal anti-Phospho-GSK-3 beta (Ser9) (5B3) Cell Signaling Technology Cat#9323; RRID:AB_2115201 Rabbit monoclonal anti-Cyclin D1 (E3P5S) Cell Signaling Technology Cat#55506; RRID:AB_2827374 Rabbit polyclonal anti-c-MYC Proteintech Cat#10828-1-AP; RRID:AB_2148585 Rabbit monoclonal anti-Phospho-Histone H2A.X (Ser139) (20E3) Cell Signaling Technology Cat#9718; RRID:AB_2118009 Rabbit polyclonal anti-TOP1 Proteintech Cat#20705-1-AP; RRID:AB_10695762 Rabbit polyclonal anti-TCF1 (C63D9) Cell Signaling Technology Cat#2203; RRID:AB_2199302 HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat#RGAR001; RRID:AB_3073505 HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat#RGAM001; RRID:AB_3068333 Biological samples CRC patient samples This paper Table S1 Chemicals, peptides, and recombinant proteins IMMU132 Gilead Sciences Cat#S22F026D Capecitabine Selleck Cat#S1156 Oxaliplatin Selleck Cat#S1224 GSK2606414 Selleck Cat#S7307 GSK2656157 Selleck Cat#S7033 AMG44 Selleck Cat#E1761 PERK-IN-4 MedChemExpress Cat#1337531-89-1 MSAB Selleck Cat#S6901 SKL2001 Selleck Cat#S8320 Polybrene YEASEN Cat#40804ES76 Puromycin Beyotime Cat#ST551 SDS Lysis Buffer Beyotime Cat#P0013G PMSF Solarbio Cat#P0100 Phosphatase inhibitor cocktail Beyotime Cat#P1081 (Continued on next page) Cell Reports Medicine 7, 102769, May 19, 2026 e1 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-fade Mounting Medium with DAPI Beyotime Cat#P0131 Critical commercial assays CellTiter-Glo® Luminescent Cell Viability Assay Promega Cat#G7573 EdU Cell Proliferation Kit with Alexa Fluor 555 EpiZyme Cat#CX003 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062L UltraSensitiveTM SP(mouse/rabbit)IHC Kit MXB Biotechnologies Cat#KIT-9730 TransZol Up TransGen Biotech Cat#ET111 EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech Cat#AE311 PerfectStart® Green qPCR SuperMix TransGen Biotech Cat#AQ602 Colorectal Cancer Organoid Kit BioGenous Technologies Cat#K2103-CR Tumor Tissue Digestion Solution BioGenous Technologies Cat#K601003 Cancer Organoid Basal Medium BioGenous Technologies Cat#B213152 Organoid Culture ECM BioGenous Technologies Cat#M315066 Organoid Dissociation Solution BioGenous Technologies Cat#E238001 Alanine aminotransferase Assay Kit Jiancheng Bioengineering Cat#C009-2-1 Aspartate aminotransferase Assay Kit Jiancheng Bioengineering Cat#C010-2-1 Urea Assay Kit Jiancheng Bioengineering Cat#C013-2-1 Uric acid (UA) Test Kit Jiancheng Bioengineering Cat#C012-2-1 Creatinine (Cr) Assay kit (sarcosine oxidase) Jiancheng Bioengineering CatC011-2-1 α-Amylase Assay Kit Jiancheng Bioengineering Cat#C016-1-2 Lipase assay kit Jiancheng Bioengineering Cat#A054-2-1 Deposited data PDX RNAseq This paper GEO: GSE311016 Cell line RNAseq This paper GEO: GSE312098 Experimental models: Cell lines Human:CX-1 iCell Bioscience Inc. iCell-h050 Human:DLD-1 iCell Bioscience Inc. iCell-h053 Human:HCT15 iCell Bioscience Inc. iCell-h072 Human:COLO205 iCell Bioscience Inc. iCell-h045 Human:HEK293T iCell Bioscience Inc. iCell-h237 Experimental models: Organisms/strains Mouse: NOD/SCID mice females Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Mouse: C57BL/6 mice males Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Oligonucleotides ShPERK#1 targeted sequence: 5′- GAAACAGCTATTCTCATAAAG -3′ Sangon Biotech N/A ShPERK #2 targeted sequence: 5′- GTTGTGCTAGCAACCCTAATA -3′ Sangon Biotech N/A Recombinant DNA pLKO.1 This paper N/A plasmids pMD2.G This paper Addgene plasmid #12259 Plasmids psPAX2 This paper Addgene plasmid #12260 Software and algorithms GraphPad Prism 10.1.2 Graphpad software https://www.graphpad.com ImageJ This paper https://imagej.net/ij/. (Continued on next page) e2 Cell Reports Medicine 7, 102769, May 19, 2026 Article ll OPEN ACCESS ..

RNA sequencing:

Article Title: Overcoming ADC resistance in advanced colorectal cancer by dual targeting of TROP2 and PERK to suppress Wnt/β-catenin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-TROP2 Abcam Cat#AB214488; RRID:AB_2811182 Rabbit monoclonal anti-Ki67 Cell Signaling Technology Cat#9027; RRID:AB_2636984 Rabbit monoclonal anti-PERK Cell Signaling Technology Cat#5683; RRID:AB_10841299 Rabbit monoclonal anti-Phospho-PERK/ EIF2AK3 Proteintech Cat#82534-1-RR; RRID:AB_3085359 Rabbit monoclonal anti-eIF2α Proteintech Cat#11170-1-AP; RRID:AB_2096489 Rabbit monoclonal anti-Phospho-eIF2α Proteintech Cat#68023-1-Ig; RRID:AB_2918767 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat#11815S; RRID:AB_2616025 Rabbit monoclonal anti-β-Catenin Proteintech Cat#51067-2-AP; RRID:AB_2086128 Rabbit polyclonal anti-Jun Proteintech Cat#24909-1-AP; RRID:AB_2860574 Mouse polyclonal anti-GSK3B Proteintech Cat#67329-1-Ig; RRID:AB_2882588 Rabbit polyclonal anti-WNT2B ABclonal Cat#A19554; RRID:AB_3741590 Rabbit monoclonal anti-GAPDH Proteintech Cat#81640-5-RR; RRID:AB_3086559 Rabbit monoclonal anti-Phospho-Beta Catenin (Ser33) Proteintech Cat#80067-1-RR; RRID:AB_2918861 Rabbit monoclonal anti-Phospho-GSK-3 beta (Ser9) (5B3) Cell Signaling Technology Cat#9323; RRID:AB_2115201 Rabbit monoclonal anti-Cyclin D1 (E3P5S) Cell Signaling Technology Cat#55506; RRID:AB_2827374 Rabbit polyclonal anti-c-MYC Proteintech Cat#10828-1-AP; RRID:AB_2148585 Rabbit monoclonal anti-Phospho-Histone H2A.X (Ser139) (20E3) Cell Signaling Technology Cat#9718; RRID:AB_2118009 Rabbit polyclonal anti-TOP1 Proteintech Cat#20705-1-AP; RRID:AB_10695762 Rabbit polyclonal anti-TCF1 (C63D9) Cell Signaling Technology Cat#2203; RRID:AB_2199302 HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat#RGAR001; RRID:AB_3073505 HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat#RGAM001; RRID:AB_3068333 Biological samples CRC patient samples This paper Table S1 Chemicals, peptides, and recombinant proteins IMMU132 Gilead Sciences Cat#S22F026D Capecitabine Selleck Cat#S1156 Oxaliplatin Selleck Cat#S1224 GSK2606414 Selleck Cat#S7307 GSK2656157 Selleck Cat#S7033 AMG44 Selleck Cat#E1761 PERK-IN-4 MedChemExpress Cat#1337531-89-1 MSAB Selleck Cat#S6901 SKL2001 Selleck Cat#S8320 Polybrene YEASEN Cat#40804ES76 Puromycin Beyotime Cat#ST551 SDS Lysis Buffer Beyotime Cat#P0013G PMSF Solarbio Cat#P0100 Phosphatase inhibitor cocktail Beyotime Cat#P1081 (Continued on next page) Cell Reports Medicine 7, 102769, May 19, 2026 e1 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-fade Mounting Medium with DAPI Beyotime Cat#P0131 Critical commercial assays CellTiter-Glo® Luminescent Cell Viability Assay Promega Cat#G7573 EdU Cell Proliferation Kit with Alexa Fluor 555 EpiZyme Cat#CX003 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062L UltraSensitiveTM SP(mouse/rabbit)IHC Kit MXB Biotechnologies Cat#KIT-9730 TransZol Up TransGen Biotech Cat#ET111 EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech Cat#AE311 PerfectStart® Green qPCR SuperMix TransGen Biotech Cat#AQ602 Colorectal Cancer Organoid Kit BioGenous Technologies Cat#K2103-CR Tumor Tissue Digestion Solution BioGenous Technologies Cat#K601003 Cancer Organoid Basal Medium BioGenous Technologies Cat#B213152 Organoid Culture ECM BioGenous Technologies Cat#M315066 Organoid Dissociation Solution BioGenous Technologies Cat#E238001 Alanine aminotransferase Assay Kit Jiancheng Bioengineering Cat#C009-2-1 Aspartate aminotransferase Assay Kit Jiancheng Bioengineering Cat#C010-2-1 Urea Assay Kit Jiancheng Bioengineering Cat#C013-2-1 Uric acid (UA) Test Kit Jiancheng Bioengineering Cat#C012-2-1 Creatinine (Cr) Assay kit (sarcosine oxidase) Jiancheng Bioengineering CatC011-2-1 α-Amylase Assay Kit Jiancheng Bioengineering Cat#C016-1-2 Lipase assay kit Jiancheng Bioengineering Cat#A054-2-1 Deposited data PDX RNAseq This paper GEO: GSE311016 Cell line RNAseq This paper GEO: GSE312098 Experimental models: Cell lines Human:CX-1 iCell Bioscience Inc. iCell-h050 Human:DLD-1 iCell Bioscience Inc. iCell-h053 Human:HCT15 iCell Bioscience Inc. iCell-h072 Human:COLO205 iCell Bioscience Inc. iCell-h045 Human:HEK293T iCell Bioscience Inc. iCell-h237 Experimental models: Organisms/strains Mouse: NOD/SCID mice females Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Mouse: C57BL/6 mice males Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Oligonucleotides ShPERK#1 targeted sequence: 5′- GAAACAGCTATTCTCATAAAG -3′ Sangon Biotech N/A ShPERK #2 targeted sequence: 5′- GTTGTGCTAGCAACCCTAATA -3′ Sangon Biotech N/A Recombinant DNA pLKO.1 This paper N/A plasmids pMD2.G This paper Addgene plasmid #12259 Plasmids psPAX2 This paper Addgene plasmid #12260 Software and algorithms GraphPad Prism 10.1.2 Graphpad software https://www.graphpad.com ImageJ This paper https://imagej.net/ij/. (Continued on next page) e2 Cell Reports Medicine 7, 102769, May 19, 2026 Article ll OPEN ACCESS ..

Sequencing:

Article Title: Overcoming ADC resistance in advanced colorectal cancer by dual targeting of TROP2 and PERK to suppress Wnt/β-catenin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-TROP2 Abcam Cat#AB214488; RRID:AB_2811182 Rabbit monoclonal anti-Ki67 Cell Signaling Technology Cat#9027; RRID:AB_2636984 Rabbit monoclonal anti-PERK Cell Signaling Technology Cat#5683; RRID:AB_10841299 Rabbit monoclonal anti-Phospho-PERK/ EIF2AK3 Proteintech Cat#82534-1-RR; RRID:AB_3085359 Rabbit monoclonal anti-eIF2α Proteintech Cat#11170-1-AP; RRID:AB_2096489 Rabbit monoclonal anti-Phospho-eIF2α Proteintech Cat#68023-1-Ig; RRID:AB_2918767 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat#11815S; RRID:AB_2616025 Rabbit monoclonal anti-β-Catenin Proteintech Cat#51067-2-AP; RRID:AB_2086128 Rabbit polyclonal anti-Jun Proteintech Cat#24909-1-AP; RRID:AB_2860574 Mouse polyclonal anti-GSK3B Proteintech Cat#67329-1-Ig; RRID:AB_2882588 Rabbit polyclonal anti-WNT2B ABclonal Cat#A19554; RRID:AB_3741590 Rabbit monoclonal anti-GAPDH Proteintech Cat#81640-5-RR; RRID:AB_3086559 Rabbit monoclonal anti-Phospho-Beta Catenin (Ser33) Proteintech Cat#80067-1-RR; RRID:AB_2918861 Rabbit monoclonal anti-Phospho-GSK-3 beta (Ser9) (5B3) Cell Signaling Technology Cat#9323; RRID:AB_2115201 Rabbit monoclonal anti-Cyclin D1 (E3P5S) Cell Signaling Technology Cat#55506; RRID:AB_2827374 Rabbit polyclonal anti-c-MYC Proteintech Cat#10828-1-AP; RRID:AB_2148585 Rabbit monoclonal anti-Phospho-Histone H2A.X (Ser139) (20E3) Cell Signaling Technology Cat#9718; RRID:AB_2118009 Rabbit polyclonal anti-TOP1 Proteintech Cat#20705-1-AP; RRID:AB_10695762 Rabbit polyclonal anti-TCF1 (C63D9) Cell Signaling Technology Cat#2203; RRID:AB_2199302 HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat#RGAR001; RRID:AB_3073505 HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat#RGAM001; RRID:AB_3068333 Biological samples CRC patient samples This paper Table S1 Chemicals, peptides, and recombinant proteins IMMU132 Gilead Sciences Cat#S22F026D Capecitabine Selleck Cat#S1156 Oxaliplatin Selleck Cat#S1224 GSK2606414 Selleck Cat#S7307 GSK2656157 Selleck Cat#S7033 AMG44 Selleck Cat#E1761 PERK-IN-4 MedChemExpress Cat#1337531-89-1 MSAB Selleck Cat#S6901 SKL2001 Selleck Cat#S8320 Polybrene YEASEN Cat#40804ES76 Puromycin Beyotime Cat#ST551 SDS Lysis Buffer Beyotime Cat#P0013G PMSF Solarbio Cat#P0100 Phosphatase inhibitor cocktail Beyotime Cat#P1081 (Continued on next page) Cell Reports Medicine 7, 102769, May 19, 2026 e1 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-fade Mounting Medium with DAPI Beyotime Cat#P0131 Critical commercial assays CellTiter-Glo® Luminescent Cell Viability Assay Promega Cat#G7573 EdU Cell Proliferation Kit with Alexa Fluor 555 EpiZyme Cat#CX003 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062L UltraSensitiveTM SP(mouse/rabbit)IHC Kit MXB Biotechnologies Cat#KIT-9730 TransZol Up TransGen Biotech Cat#ET111 EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech Cat#AE311 PerfectStart® Green qPCR SuperMix TransGen Biotech Cat#AQ602 Colorectal Cancer Organoid Kit BioGenous Technologies Cat#K2103-CR Tumor Tissue Digestion Solution BioGenous Technologies Cat#K601003 Cancer Organoid Basal Medium BioGenous Technologies Cat#B213152 Organoid Culture ECM BioGenous Technologies Cat#M315066 Organoid Dissociation Solution BioGenous Technologies Cat#E238001 Alanine aminotransferase Assay Kit Jiancheng Bioengineering Cat#C009-2-1 Aspartate aminotransferase Assay Kit Jiancheng Bioengineering Cat#C010-2-1 Urea Assay Kit Jiancheng Bioengineering Cat#C013-2-1 Uric acid (UA) Test Kit Jiancheng Bioengineering Cat#C012-2-1 Creatinine (Cr) Assay kit (sarcosine oxidase) Jiancheng Bioengineering CatC011-2-1 α-Amylase Assay Kit Jiancheng Bioengineering Cat#C016-1-2 Lipase assay kit Jiancheng Bioengineering Cat#A054-2-1 Deposited data PDX RNAseq This paper GEO: GSE311016 Cell line RNAseq This paper GEO: GSE312098 Experimental models: Cell lines Human:CX-1 iCell Bioscience Inc. iCell-h050 Human:DLD-1 iCell Bioscience Inc. iCell-h053 Human:HCT15 iCell Bioscience Inc. iCell-h072 Human:COLO205 iCell Bioscience Inc. iCell-h045 Human:HEK293T iCell Bioscience Inc. iCell-h237 Experimental models: Organisms/strains Mouse: NOD/SCID mice females Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Mouse: C57BL/6 mice males Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Oligonucleotides ShPERK#1 targeted sequence: 5′- GAAACAGCTATTCTCATAAAG -3′ Sangon Biotech N/A ShPERK #2 targeted sequence: 5′- GTTGTGCTAGCAACCCTAATA -3′ Sangon Biotech N/A Recombinant DNA pLKO.1 This paper N/A plasmids pMD2.G This paper Addgene plasmid #12259 Plasmids psPAX2 This paper Addgene plasmid #12260 Software and algorithms GraphPad Prism 10.1.2 Graphpad software https://www.graphpad.com ImageJ This paper https://imagej.net/ij/. (Continued on next page) e2 Cell Reports Medicine 7, 102769, May 19, 2026 Article ll OPEN ACCESS ..

Recombinant:

Article Title: Overcoming ADC resistance in advanced colorectal cancer by dual targeting of TROP2 and PERK to suppress Wnt/β-catenin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-TROP2 Abcam Cat#AB214488; RRID:AB_2811182 Rabbit monoclonal anti-Ki67 Cell Signaling Technology Cat#9027; RRID:AB_2636984 Rabbit monoclonal anti-PERK Cell Signaling Technology Cat#5683; RRID:AB_10841299 Rabbit monoclonal anti-Phospho-PERK/ EIF2AK3 Proteintech Cat#82534-1-RR; RRID:AB_3085359 Rabbit monoclonal anti-eIF2α Proteintech Cat#11170-1-AP; RRID:AB_2096489 Rabbit monoclonal anti-Phospho-eIF2α Proteintech Cat#68023-1-Ig; RRID:AB_2918767 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat#11815S; RRID:AB_2616025 Rabbit monoclonal anti-β-Catenin Proteintech Cat#51067-2-AP; RRID:AB_2086128 Rabbit polyclonal anti-Jun Proteintech Cat#24909-1-AP; RRID:AB_2860574 Mouse polyclonal anti-GSK3B Proteintech Cat#67329-1-Ig; RRID:AB_2882588 Rabbit polyclonal anti-WNT2B ABclonal Cat#A19554; RRID:AB_3741590 Rabbit monoclonal anti-GAPDH Proteintech Cat#81640-5-RR; RRID:AB_3086559 Rabbit monoclonal anti-Phospho-Beta Catenin (Ser33) Proteintech Cat#80067-1-RR; RRID:AB_2918861 Rabbit monoclonal anti-Phospho-GSK-3 beta (Ser9) (5B3) Cell Signaling Technology Cat#9323; RRID:AB_2115201 Rabbit monoclonal anti-Cyclin D1 (E3P5S) Cell Signaling Technology Cat#55506; RRID:AB_2827374 Rabbit polyclonal anti-c-MYC Proteintech Cat#10828-1-AP; RRID:AB_2148585 Rabbit monoclonal anti-Phospho-Histone H2A.X (Ser139) (20E3) Cell Signaling Technology Cat#9718; RRID:AB_2118009 Rabbit polyclonal anti-TOP1 Proteintech Cat#20705-1-AP; RRID:AB_10695762 Rabbit polyclonal anti-TCF1 (C63D9) Cell Signaling Technology Cat#2203; RRID:AB_2199302 HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat#RGAR001; RRID:AB_3073505 HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat#RGAM001; RRID:AB_3068333 Biological samples CRC patient samples This paper Table S1 Chemicals, peptides, and recombinant proteins IMMU132 Gilead Sciences Cat#S22F026D Capecitabine Selleck Cat#S1156 Oxaliplatin Selleck Cat#S1224 GSK2606414 Selleck Cat#S7307 GSK2656157 Selleck Cat#S7033 AMG44 Selleck Cat#E1761 PERK-IN-4 MedChemExpress Cat#1337531-89-1 MSAB Selleck Cat#S6901 SKL2001 Selleck Cat#S8320 Polybrene YEASEN Cat#40804ES76 Puromycin Beyotime Cat#ST551 SDS Lysis Buffer Beyotime Cat#P0013G PMSF Solarbio Cat#P0100 Phosphatase inhibitor cocktail Beyotime Cat#P1081 (Continued on next page) Cell Reports Medicine 7, 102769, May 19, 2026 e1 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-fade Mounting Medium with DAPI Beyotime Cat#P0131 Critical commercial assays CellTiter-Glo® Luminescent Cell Viability Assay Promega Cat#G7573 EdU Cell Proliferation Kit with Alexa Fluor 555 EpiZyme Cat#CX003 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062L UltraSensitiveTM SP(mouse/rabbit)IHC Kit MXB Biotechnologies Cat#KIT-9730 TransZol Up TransGen Biotech Cat#ET111 EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech Cat#AE311 PerfectStart® Green qPCR SuperMix TransGen Biotech Cat#AQ602 Colorectal Cancer Organoid Kit BioGenous Technologies Cat#K2103-CR Tumor Tissue Digestion Solution BioGenous Technologies Cat#K601003 Cancer Organoid Basal Medium BioGenous Technologies Cat#B213152 Organoid Culture ECM BioGenous Technologies Cat#M315066 Organoid Dissociation Solution BioGenous Technologies Cat#E238001 Alanine aminotransferase Assay Kit Jiancheng Bioengineering Cat#C009-2-1 Aspartate aminotransferase Assay Kit Jiancheng Bioengineering Cat#C010-2-1 Urea Assay Kit Jiancheng Bioengineering Cat#C013-2-1 Uric acid (UA) Test Kit Jiancheng Bioengineering Cat#C012-2-1 Creatinine (Cr) Assay kit (sarcosine oxidase) Jiancheng Bioengineering CatC011-2-1 α-Amylase Assay Kit Jiancheng Bioengineering Cat#C016-1-2 Lipase assay kit Jiancheng Bioengineering Cat#A054-2-1 Deposited data PDX RNAseq This paper GEO: GSE311016 Cell line RNAseq This paper GEO: GSE312098 Experimental models: Cell lines Human:CX-1 iCell Bioscience Inc. iCell-h050 Human:DLD-1 iCell Bioscience Inc. iCell-h053 Human:HCT15 iCell Bioscience Inc. iCell-h072 Human:COLO205 iCell Bioscience Inc. iCell-h045 Human:HEK293T iCell Bioscience Inc. iCell-h237 Experimental models: Organisms/strains Mouse: NOD/SCID mice females Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Mouse: C57BL/6 mice males Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Oligonucleotides ShPERK#1 targeted sequence: 5′- GAAACAGCTATTCTCATAAAG -3′ Sangon Biotech N/A ShPERK #2 targeted sequence: 5′- GTTGTGCTAGCAACCCTAATA -3′ Sangon Biotech N/A Recombinant DNA pLKO.1 This paper N/A plasmids pMD2.G This paper Addgene plasmid #12259 Plasmids psPAX2 This paper Addgene plasmid #12260 Software and algorithms GraphPad Prism 10.1.2 Graphpad software https://www.graphpad.com ImageJ This paper https://imagej.net/ij/. (Continued on next page) e2 Cell Reports Medicine 7, 102769, May 19, 2026 Article ll OPEN ACCESS ..

Plasmid Preparation:

Article Title: Overcoming ADC resistance in advanced colorectal cancer by dual targeting of TROP2 and PERK to suppress Wnt/β-catenin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-TROP2 Abcam Cat#AB214488; RRID:AB_2811182 Rabbit monoclonal anti-Ki67 Cell Signaling Technology Cat#9027; RRID:AB_2636984 Rabbit monoclonal anti-PERK Cell Signaling Technology Cat#5683; RRID:AB_10841299 Rabbit monoclonal anti-Phospho-PERK/ EIF2AK3 Proteintech Cat#82534-1-RR; RRID:AB_3085359 Rabbit monoclonal anti-eIF2α Proteintech Cat#11170-1-AP; RRID:AB_2096489 Rabbit monoclonal anti-Phospho-eIF2α Proteintech Cat#68023-1-Ig; RRID:AB_2918767 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat#11815S; RRID:AB_2616025 Rabbit monoclonal anti-β-Catenin Proteintech Cat#51067-2-AP; RRID:AB_2086128 Rabbit polyclonal anti-Jun Proteintech Cat#24909-1-AP; RRID:AB_2860574 Mouse polyclonal anti-GSK3B Proteintech Cat#67329-1-Ig; RRID:AB_2882588 Rabbit polyclonal anti-WNT2B ABclonal Cat#A19554; RRID:AB_3741590 Rabbit monoclonal anti-GAPDH Proteintech Cat#81640-5-RR; RRID:AB_3086559 Rabbit monoclonal anti-Phospho-Beta Catenin (Ser33) Proteintech Cat#80067-1-RR; RRID:AB_2918861 Rabbit monoclonal anti-Phospho-GSK-3 beta (Ser9) (5B3) Cell Signaling Technology Cat#9323; RRID:AB_2115201 Rabbit monoclonal anti-Cyclin D1 (E3P5S) Cell Signaling Technology Cat#55506; RRID:AB_2827374 Rabbit polyclonal anti-c-MYC Proteintech Cat#10828-1-AP; RRID:AB_2148585 Rabbit monoclonal anti-Phospho-Histone H2A.X (Ser139) (20E3) Cell Signaling Technology Cat#9718; RRID:AB_2118009 Rabbit polyclonal anti-TOP1 Proteintech Cat#20705-1-AP; RRID:AB_10695762 Rabbit polyclonal anti-TCF1 (C63D9) Cell Signaling Technology Cat#2203; RRID:AB_2199302 HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat#RGAR001; RRID:AB_3073505 HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat#RGAM001; RRID:AB_3068333 Biological samples CRC patient samples This paper Table S1 Chemicals, peptides, and recombinant proteins IMMU132 Gilead Sciences Cat#S22F026D Capecitabine Selleck Cat#S1156 Oxaliplatin Selleck Cat#S1224 GSK2606414 Selleck Cat#S7307 GSK2656157 Selleck Cat#S7033 AMG44 Selleck Cat#E1761 PERK-IN-4 MedChemExpress Cat#1337531-89-1 MSAB Selleck Cat#S6901 SKL2001 Selleck Cat#S8320 Polybrene YEASEN Cat#40804ES76 Puromycin Beyotime Cat#ST551 SDS Lysis Buffer Beyotime Cat#P0013G PMSF Solarbio Cat#P0100 Phosphatase inhibitor cocktail Beyotime Cat#P1081 (Continued on next page) Cell Reports Medicine 7, 102769, May 19, 2026 e1 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-fade Mounting Medium with DAPI Beyotime Cat#P0131 Critical commercial assays CellTiter-Glo® Luminescent Cell Viability Assay Promega Cat#G7573 EdU Cell Proliferation Kit with Alexa Fluor 555 EpiZyme Cat#CX003 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062L UltraSensitiveTM SP(mouse/rabbit)IHC Kit MXB Biotechnologies Cat#KIT-9730 TransZol Up TransGen Biotech Cat#ET111 EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech Cat#AE311 PerfectStart® Green qPCR SuperMix TransGen Biotech Cat#AQ602 Colorectal Cancer Organoid Kit BioGenous Technologies Cat#K2103-CR Tumor Tissue Digestion Solution BioGenous Technologies Cat#K601003 Cancer Organoid Basal Medium BioGenous Technologies Cat#B213152 Organoid Culture ECM BioGenous Technologies Cat#M315066 Organoid Dissociation Solution BioGenous Technologies Cat#E238001 Alanine aminotransferase Assay Kit Jiancheng Bioengineering Cat#C009-2-1 Aspartate aminotransferase Assay Kit Jiancheng Bioengineering Cat#C010-2-1 Urea Assay Kit Jiancheng Bioengineering Cat#C013-2-1 Uric acid (UA) Test Kit Jiancheng Bioengineering Cat#C012-2-1 Creatinine (Cr) Assay kit (sarcosine oxidase) Jiancheng Bioengineering CatC011-2-1 α-Amylase Assay Kit Jiancheng Bioengineering Cat#C016-1-2 Lipase assay kit Jiancheng Bioengineering Cat#A054-2-1 Deposited data PDX RNAseq This paper GEO: GSE311016 Cell line RNAseq This paper GEO: GSE312098 Experimental models: Cell lines Human:CX-1 iCell Bioscience Inc. iCell-h050 Human:DLD-1 iCell Bioscience Inc. iCell-h053 Human:HCT15 iCell Bioscience Inc. iCell-h072 Human:COLO205 iCell Bioscience Inc. iCell-h045 Human:HEK293T iCell Bioscience Inc. iCell-h237 Experimental models: Organisms/strains Mouse: NOD/SCID mice females Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Mouse: C57BL/6 mice males Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Oligonucleotides ShPERK#1 targeted sequence: 5′- GAAACAGCTATTCTCATAAAG -3′ Sangon Biotech N/A ShPERK #2 targeted sequence: 5′- GTTGTGCTAGCAACCCTAATA -3′ Sangon Biotech N/A Recombinant DNA pLKO.1 This paper N/A plasmids pMD2.G This paper Addgene plasmid #12259 Plasmids psPAX2 This paper Addgene plasmid #12260 Software and algorithms GraphPad Prism 10.1.2 Graphpad software https://www.graphpad.com ImageJ This paper https://imagej.net/ij/. (Continued on next page) e2 Cell Reports Medicine 7, 102769, May 19, 2026 Article ll OPEN ACCESS ..

Software:

Article Title: Overcoming ADC resistance in advanced colorectal cancer by dual targeting of TROP2 and PERK to suppress Wnt/β-catenin signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-TROP2 Abcam Cat#AB214488; RRID:AB_2811182 Rabbit monoclonal anti-Ki67 Cell Signaling Technology Cat#9027; RRID:AB_2636984 Rabbit monoclonal anti-PERK Cell Signaling Technology Cat#5683; RRID:AB_10841299 Rabbit monoclonal anti-Phospho-PERK/ EIF2AK3 Proteintech Cat#82534-1-RR; RRID:AB_3085359 Rabbit monoclonal anti-eIF2α Proteintech Cat#11170-1-AP; RRID:AB_2096489 Rabbit monoclonal anti-Phospho-eIF2α Proteintech Cat#68023-1-Ig; RRID:AB_2918767 Rabbit monoclonal anti-ATF4 Cell Signaling Technology Cat#11815S; RRID:AB_2616025 Rabbit monoclonal anti-β-Catenin Proteintech Cat#51067-2-AP; RRID:AB_2086128 Rabbit polyclonal anti-Jun Proteintech Cat#24909-1-AP; RRID:AB_2860574 Mouse polyclonal anti-GSK3B Proteintech Cat#67329-1-Ig; RRID:AB_2882588 Rabbit polyclonal anti-WNT2B ABclonal Cat#A19554; RRID:AB_3741590 Rabbit monoclonal anti-GAPDH Proteintech Cat#81640-5-RR; RRID:AB_3086559 Rabbit monoclonal anti-Phospho-Beta Catenin (Ser33) Proteintech Cat#80067-1-RR; RRID:AB_2918861 Rabbit monoclonal anti-Phospho-GSK-3 beta (Ser9) (5B3) Cell Signaling Technology Cat#9323; RRID:AB_2115201 Rabbit monoclonal anti-Cyclin D1 (E3P5S) Cell Signaling Technology Cat#55506; RRID:AB_2827374 Rabbit polyclonal anti-c-MYC Proteintech Cat#10828-1-AP; RRID:AB_2148585 Rabbit monoclonal anti-Phospho-Histone H2A.X (Ser139) (20E3) Cell Signaling Technology Cat#9718; RRID:AB_2118009 Rabbit polyclonal anti-TOP1 Proteintech Cat#20705-1-AP; RRID:AB_10695762 Rabbit polyclonal anti-TCF1 (C63D9) Cell Signaling Technology Cat#2203; RRID:AB_2199302 HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) Proteintech Cat#RGAR001; RRID:AB_3073505 HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) Proteintech Cat#RGAM001; RRID:AB_3068333 Biological samples CRC patient samples This paper Table S1 Chemicals, peptides, and recombinant proteins IMMU132 Gilead Sciences Cat#S22F026D Capecitabine Selleck Cat#S1156 Oxaliplatin Selleck Cat#S1224 GSK2606414 Selleck Cat#S7307 GSK2656157 Selleck Cat#S7033 AMG44 Selleck Cat#E1761 PERK-IN-4 MedChemExpress Cat#1337531-89-1 MSAB Selleck Cat#S6901 SKL2001 Selleck Cat#S8320 Polybrene YEASEN Cat#40804ES76 Puromycin Beyotime Cat#ST551 SDS Lysis Buffer Beyotime Cat#P0013G PMSF Solarbio Cat#P0100 Phosphatase inhibitor cocktail Beyotime Cat#P1081 (Continued on next page) Cell Reports Medicine 7, 102769, May 19, 2026 e1 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-fade Mounting Medium with DAPI Beyotime Cat#P0131 Critical commercial assays CellTiter-Glo® Luminescent Cell Viability Assay Promega Cat#G7573 EdU Cell Proliferation Kit with Alexa Fluor 555 EpiZyme Cat#CX003 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062L UltraSensitiveTM SP(mouse/rabbit)IHC Kit MXB Biotechnologies Cat#KIT-9730 TransZol Up TransGen Biotech Cat#ET111 EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech Cat#AE311 PerfectStart® Green qPCR SuperMix TransGen Biotech Cat#AQ602 Colorectal Cancer Organoid Kit BioGenous Technologies Cat#K2103-CR Tumor Tissue Digestion Solution BioGenous Technologies Cat#K601003 Cancer Organoid Basal Medium BioGenous Technologies Cat#B213152 Organoid Culture ECM BioGenous Technologies Cat#M315066 Organoid Dissociation Solution BioGenous Technologies Cat#E238001 Alanine aminotransferase Assay Kit Jiancheng Bioengineering Cat#C009-2-1 Aspartate aminotransferase Assay Kit Jiancheng Bioengineering Cat#C010-2-1 Urea Assay Kit Jiancheng Bioengineering Cat#C013-2-1 Uric acid (UA) Test Kit Jiancheng Bioengineering Cat#C012-2-1 Creatinine (Cr) Assay kit (sarcosine oxidase) Jiancheng Bioengineering CatC011-2-1 α-Amylase Assay Kit Jiancheng Bioengineering Cat#C016-1-2 Lipase assay kit Jiancheng Bioengineering Cat#A054-2-1 Deposited data PDX RNAseq This paper GEO: GSE311016 Cell line RNAseq This paper GEO: GSE312098 Experimental models: Cell lines Human:CX-1 iCell Bioscience Inc. iCell-h050 Human:DLD-1 iCell Bioscience Inc. iCell-h053 Human:HCT15 iCell Bioscience Inc. iCell-h072 Human:COLO205 iCell Bioscience Inc. iCell-h045 Human:HEK293T iCell Bioscience Inc. iCell-h237 Experimental models: Organisms/strains Mouse: NOD/SCID mice females Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Mouse: C57BL/6 mice males Jiangsu Huachuang sino Pharma Tech Co.,Ltd N/A Oligonucleotides ShPERK#1 targeted sequence: 5′- GAAACAGCTATTCTCATAAAG -3′ Sangon Biotech N/A ShPERK #2 targeted sequence: 5′- GTTGTGCTAGCAACCCTAATA -3′ Sangon Biotech N/A Recombinant DNA pLKO.1 This paper N/A plasmids pMD2.G This paper Addgene plasmid #12259 Plasmids psPAX2 This paper Addgene plasmid #12260 Software and algorithms GraphPad Prism 10.1.2 Graphpad software https://www.graphpad.com ImageJ This paper https://imagej.net/ij/. (Continued on next page) e2 Cell Reports Medicine 7, 102769, May 19, 2026 Article ll OPEN ACCESS ..

other:

Article Title: Overcoming ADC resistance in advanced colorectal cancer by dual targeting of TROP2 and PERK to suppress Wnt/β-catenin signaling
Article Snippet: Creatinine (Cr) Assay kit (sarcosine oxidase) , Jiancheng Bioengineering , CatC011-2-1.

Centrifugation:

Article Title: LINC00114 promotes colorectal cancer metastasis by targeting HNRNPA1 to regulate glutamine metabolism reprogramming and angiogenesis.
Article Snippet: Tang et al. Acta Biochim Biophys Sin 2025 Metabolic index detection Glucose uptake, adenosine-triphosphate (ATP) content, lactate level, and pyruvate dehydrogenase (PDH) activity in CRC cells and xenograft tumor tissues were evaluated via a glucose uptake assay kit (36503; AAT Bioquest, Pleasanton, USA), ATP assay kit (A095-11; Jiancheng, Nanjing, China), lactic acid assay kit (A019-2-1; Jiancheng) and PDH assay kit (ZC-S0391; ZCIBIO, Shanghai, China) respectively. .. In addition, serum was extracted from the mice in each group via centrifugation, and the levels of ALT, AST, BUN, Cr, LDH, CK and GLU in the serum were measured using the alanine aminotransferase (ALT) assay kit (C009-2-1; Jiancheng), aspartate aminotransferase (AST) assay kit (C010-2-1; Jiancheng), urea (BUN) assay kit (C013-2-1; Jiancheng), creatinine (Cr) assay kit (C011-2-1; Jiancheng), lactate dehydrogenase (LDH) assay kit (A020-2-2; Jiancheng), creatine kinase (CK) assay kit (A032-1-1; Jiancheng), and glucose (GLU) kit (A154-1-1; Jiancheng) respectively. ..

AST Assay:

Article Title: LINC00114 promotes colorectal cancer metastasis by targeting HNRNPA1 to regulate glutamine metabolism reprogramming and angiogenesis.
Article Snippet: Tang et al. Acta Biochim Biophys Sin 2025 Metabolic index detection Glucose uptake, adenosine-triphosphate (ATP) content, lactate level, and pyruvate dehydrogenase (PDH) activity in CRC cells and xenograft tumor tissues were evaluated via a glucose uptake assay kit (36503; AAT Bioquest, Pleasanton, USA), ATP assay kit (A095-11; Jiancheng, Nanjing, China), lactic acid assay kit (A019-2-1; Jiancheng) and PDH assay kit (ZC-S0391; ZCIBIO, Shanghai, China) respectively. .. In addition, serum was extracted from the mice in each group via centrifugation, and the levels of ALT, AST, BUN, Cr, LDH, CK and GLU in the serum were measured using the alanine aminotransferase (ALT) assay kit (C009-2-1; Jiancheng), aspartate aminotransferase (AST) assay kit (C010-2-1; Jiancheng), urea (BUN) assay kit (C013-2-1; Jiancheng), creatinine (Cr) assay kit (C011-2-1; Jiancheng), lactate dehydrogenase (LDH) assay kit (A020-2-2; Jiancheng), creatine kinase (CK) assay kit (A032-1-1; Jiancheng), and glucose (GLU) kit (A154-1-1; Jiancheng) respectively. ..

Lactate Dehydrogenase Assay:

Article Title: LINC00114 promotes colorectal cancer metastasis by targeting HNRNPA1 to regulate glutamine metabolism reprogramming and angiogenesis.
Article Snippet: Tang et al. Acta Biochim Biophys Sin 2025 Metabolic index detection Glucose uptake, adenosine-triphosphate (ATP) content, lactate level, and pyruvate dehydrogenase (PDH) activity in CRC cells and xenograft tumor tissues were evaluated via a glucose uptake assay kit (36503; AAT Bioquest, Pleasanton, USA), ATP assay kit (A095-11; Jiancheng, Nanjing, China), lactic acid assay kit (A019-2-1; Jiancheng) and PDH assay kit (ZC-S0391; ZCIBIO, Shanghai, China) respectively. .. In addition, serum was extracted from the mice in each group via centrifugation, and the levels of ALT, AST, BUN, Cr, LDH, CK and GLU in the serum were measured using the alanine aminotransferase (ALT) assay kit (C009-2-1; Jiancheng), aspartate aminotransferase (AST) assay kit (C010-2-1; Jiancheng), urea (BUN) assay kit (C013-2-1; Jiancheng), creatinine (Cr) assay kit (C011-2-1; Jiancheng), lactate dehydrogenase (LDH) assay kit (A020-2-2; Jiancheng), creatine kinase (CK) assay kit (A032-1-1; Jiancheng), and glucose (GLU) kit (A154-1-1; Jiancheng) respectively. ..



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